MHC-PepSeq
A Novel Platform for Highly-multiplexed Proteomics
Want to obtain detailed genomic information efficiently with high-throughput sequencing readouts? That's where PepSeq comes in. We're currently applying PepSeq to our T cell/Tuberculosis work and to our SARS-CoV-2 investigation.
How it Works
PepSeq uses DNA barcodes and next-generation sequencing to dramatically increase the capacity of protein binding assays. This platform leverages the highly parallel tools of DNA writing (array-based synthesis) and reading (high-throughput digital sequencing) into the
proteomic sphere. In particular, the platform enables fully-definable libraries of 100,000s of peptides to be generated cost-effectively in one-pot reactions and then assayed in multiplex against immunological targets. Library generation takes advantage of in vitro transcription and translation of DNA templates, followed by intramolecular coupling to generate biologically-synthesized peptides that are individually covalently linked to DNA tags that enable readout by sequencing.
The Sky's the Limit
All synthesis steps occur in multiplexed single-tube reactions, meaning that the scale of the assay is limited only by the cost of DNA synthesis and sequencing.
DNA - RNA - Data
(a) The PepSeq platform enables genome data to be converted into large libraries of DNA-barcoded peptides. (b) Library preparation begins with parallel synthesis of peptide-encoding DNA templates, followed by in vitro transcription and translation steps in which multiplexed intramolecular coupling is used to generate covalently linked DNA:peptide conjugates.