Want to obtain detailed genomic information efficiently with high-throughput sequencing readouts? That's where PepSeq comes in. We're currently applying PepSeq to our T cell/Tuberculosis work and to our SARS-CoV-2 investigation.
PepSeq uses DNA barcodes and next-generation sequencing to dramatically increase the capacity of protein binding assays. This platform leverages the highly parallel tools of DNA writing (array-based synthesis) and reading (high-throughput digital sequencing) into the
proteomic sphere. In particular, the platform enables fully-definable libraries of 100,000s of peptides to be generated cost-effectively in one-pot reactions and then assayed in multiplex against immunological targets. Library generation takes advantage of in vitro transcription and translation of DNA templates, followed by intramolecular coupling to generate biologically-synthesized peptides that are individually covalently linked to DNA tags that enable readout by sequencing.
All synthesis steps occur in multiplexed single-tube reactions, meaning that the scale of the assay is limited only by the cost of DNA synthesis and sequencing.
(a) The PepSeq platform enables genome data to be converted into large libraries of DNA-barcoded peptides. (b) Library preparation begins with parallel synthesis of peptide-encoding DNA templates, followed by in vitro transcription and translation steps in which multiplexed intramolecular coupling is used to generate covalently linked DNA:peptide conjugates.